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- BioGems
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- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
RM134L
- 标记物：
Biotin
- 规格：
100 ug
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文献和实验Biotin labeling and hybridization of probe to chromosomes
Total reaction volume 20 µl The amount of the sheared blocking DNA is the most critical part of this procedure. The blocking DNA should be in excess of 100 to300
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
Preparation and purification of biotin-labeled probe for ISH
is collected in the 1.5 ml tube The final volume of probe should be close to 55 µl. Usually, 5 µl of such a biotin-labeled probe (1 µg/55 µl = 18 ng/µl) is enough for one slide, and the probe can be used to process 100 slides. The size of the probe
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