Anti-Mouse CD127 (IL-7Ra) Biot

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained

血管里的“斑块清洁工”来了！CD25+调节性T细胞变身CAR-Treg，效果惊人

调节性T细胞（Treg）是维持免疫耐受和抑制过度炎症反应的核心细胞亚群，其中CD4+CD25+Treg细胞因其独特的免疫抑制功能而备受关注。CD25（即IL-2受体α链，IL2RA）作为Treg细胞最特异性的表面标志物，不仅介导IL-2信号以维持Treg的发育与功能，也成为免疫调控研究和细胞治疗开发的关键靶点。近年来，基于Treg的细胞疗法已从肿瘤和自身免疫病拓展至心血管疾病领域。2025年发表于《Circulation》的一项突破性研究首次报道了靶向氧化低密度脂蛋白（OxLDL）的CAR

Dual- and Triple-Color Fluorospot

in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb