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- 2025年07月10日
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- 克隆性：
R4-6A2
- 标记物：
SAFIRE Purified
- 规格：
50ug
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文献和实验Th17 Polarization of Mouse CD4 Cells
Anti-mouse CD28, clone37.51[Details: LEAF™format, cat.#102112] Anti-mouse IL-4 [Details: LEAF™format, cat#504108] Anti-mouse IFN-γ [Details: LEAF™format, cat.#505812] Recombinant mouse IL-6(carrier-free)[Details: Biolengend, cat
Intracellular Cytokine Staining Protocol
by flow cytometry since cytokine levels are typically too low in freshly-prepared cells. Stimulation of cells with the appropriate reagent will depend on the cell type and the experimental conditions. For example, to stimulate T cells to produce IFN-gamma
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
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