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- BioGems
- 04111-25-50
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
RPA-2.10
- 标记物：
SAFIRE Purified
- 规格：
50 ug
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文献和实验Purification of CD2 1) Cells are pelleted (~3 - 4000 g for 10 min.) and resuspended in pre-chilled 50 mM malonate (pH 5.2-5.3) with 1mM EDTA (~40ml of buffer per litre of culture). 2) Lyse the cells with French
Isolation of a Purified Epithelial Cell Population from Human Colon
. For the human colon, isolation techniques to allow direct study have been described for mononuclear cells in the lamina propria, smooth muscle cells at or below the muscularis mucosae, and cells of the enteric nervous system, located between the subserosa
and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
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