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- BioGems
- 08212-30-100
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
2.4G2
- 标记物：
Biotin
- 规格：
100 ug
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文献和实验Biotin labeling and hybridization of probe to chromosomes
quality) at 70 C for 2 min. Immediately transfer the slides into an ethanol series (70 %, 95 %, and 100 %, 5 min each at 20 C), and air dry the slides. Add 10 µl of the hybridization mixture to each slide and cover with an '18 x 18' mm
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
DAY 1: 1) Reprecipitate DNA's Add the following DNA's to a 1.5 ml centrifuge tube, mixing with pipet: 20 ug Cot-1 DNA (~20ul) ~200 ng Biotin labeled DNA (~10ul) ~200 ng Digoxigenin labeled DNA (~10ul) Add 1/10th
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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