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- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
2.4G2
- 标记物：
Biotin
- 规格：
25 ug
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文献和实验CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
DAY 1: 1) Reprecipitate DNA's Add the following DNA's to a 1.5 ml centrifuge tube, mixing with pipet: 20 ug Cot-1 DNA (~20ul) ~200 ng Biotin labeled DNA (~10ul) ~200 ng Digoxigenin labeled DNA (~10ul) Add 1/10th
3.75ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 21.45ml H20 ChIP wash buffer 30ml final volume: 150µl 2M Tris 1.7ml 4.4M LiCl 60µl 0.5M EDTA 1.5ml 10% NAD 1.5ml 10% NP-40 25.09ml
40, then again with PBS/0.1 BSA. Mount solution (70 glycerol containing 2 n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20oC until examination. Antibodies mouse
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