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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Mouse, Monoclonal/IgG1, Kappa
- 形态:
液体
- 保存条件:
2-8℃
- 克隆性:
单克隆
- 适应物种:
Human; Rat; Not react with Mouse;
- 保质期:
一年
- 级别:
病理级
- 库存:
100
- 供应商:
ImmunoWay Biotechnology Company
- 宿主:
小鼠
- 应用范围:
IHC
- 规格:
3ml/6ml/10ml
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文献和实验pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times
. Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer . Discard Sup. Resuspend pellets in 10 ml of 100 mM Tris buffer or Binding buffer . And pool into 50 ml Falcon tubes (try not to make bubbles). Use 3.2 ml/cm 12-14
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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