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文献和实验of pluripotent P19 cells into skeletal muscle lineage by using histone deacetylase inhibitor, valproic acid and the ligand of retinoic acid receptor, all-trans retinoic acid.
concentration is low. BSA is also used to reduce the inhibition caused by humic acid contaminants. 2、Elisa中的blocking buffer 、tween、BSA 等问题 请问tween是降低非特异性binding的吗? 如果blockingbuffer中加入了0.05%的tween,会不会把之前coating上的蛋白也给洗掉了? 一抗和二抗的稀释液有什么要求? 里面的BSA
Generation of iPS Cells from Human Skin Biopsy
human fibroblasts from skin biopsies using retroviral transduction of OCT4, SOX2, KLF4, and c-MYC with the addition of the histone deacetylase (HDAC) inhibitor, valproic acid (VPA). Characterized patient-specific iPS cell lines can be obtained
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