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文献和实验Preparation Of Peptide-KLH Conjugates For Immunization
.(6) For the -SH coupling, warm the other aliquot of KLH to room temp. Add 1/9 th volume of Iodoacetic acid N-hydroxysuccinimide ester at 100mg/ml in DMSO. Make the DMSO stock fresh, and protect the iodoacetamide reagent from light. We make our own IAA-NHS ester
of pluripotent P19 cells into skeletal muscle lineage by using histone deacetylase inhibitor, valproic acid and the ligand of retinoic acid receptor, all-trans retinoic acid.
Preparation Of Peptide-KLH Conjugates For Immunization
A. Checking Peptide Thiol Groups Do this before thiol coupling, either to KLH or to resin. Peptide thiol groups have a tendency to get lost after synthesis. Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.
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