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文献和实验Mouse B cell isolation with magnetic beads
ml conical tube; 6.Centrifuge cells at 1700 rpm(500 g), 5 min, 4°C; 7. Remove red blood cell : Resuspend cells in ACK to 1 ml/1X10 8 cells, and stand in RT for 1 min. Constant volume to 20 ml/ 1X10 8 cells
Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads
that are involved in pathological conditions such as cancer (8 –10 ). Such methods are suitable for the isolation of low-expression genes. The principle of these approaches is to remove the mRNA species common to different cell types or tissues by subtraction
Amino-allyl Reverse Transcription
min. 1x dNTPS: 500uM each dATP, dCTP, dGTP, 200uM aadUTP, 300uM dTTP 50x recipe: FOR 2:3 10uL each 100mM dATP,dGTP,dCTP, 4uL 100mM aa-dUTP*, 6uL 100 mMdTTP * 5-(3-Aminoallyl)-2'-deoxyuridine 5'-triphosphate
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