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文献和实验for 2hrs c) to digest w/ NcoI, add: 1ul Nco I (10U/ul) d) digest at 37C for 2 hours e) collect PMPs with magnetic stand, and remove the 40 ul of supernatent (Ligation template) to a new tube. The digestion should liberate the cDNA from the beads
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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