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文献和实验Mouse B cell isolation with magnetic beads
ml conical tube; 6.Centrifuge cells at 1700 rpm(500 g), 5 min, 4°C; 7. Remove red blood cell : Resuspend cells in ACK to 1 ml/1X10 8 cells, and stand in RT for 1 min. Constant volume to 20 ml/ 1X10 8 cells
Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads
of specific sequences (9 ). In the last 20 yr, several subtractive strategies have been described (2 –5 ,9 ), but subtraction hybridization/cloning still remains a technically demanding, time-consuming, and labor-intensive technology, including
of protein knockdown, it is necessary to purify the transfected cells. This can be done by cotransfection of a plasmid encoding the cell surface marker protein, CD19 or CD20, followed by immunopurification of the CD19- or CD20-expressing cells with magnetic
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