Recombinant Mouse IL25/IL17E,

Recombinant DNA Engineering, Or Cloning Genes In Plasmids

also usually have promoter segments for phage polymerases (like T7 and SP6 RNA polymerases) such that one can use in them vitro to produce RNA. A plasmid designed for mammalian expression also usually has a transcription termination and polyadenylation signal

High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli

/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time

Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells

RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).