HyperChrom DEAE HP Agarose-Hyp

DNA Isolation from Agarose Gels with DEAE Paper

with the voltage increased to 100-120 V. The DEAE paper is removed and binding of the DNA checked by viewing the paper with long wave UV light.The paper is then placed in an eppendorf tube containing 500 μl  NET buffer to wash away remaining agarose.The bound DNA

ISOLATION OF DNA FROM AGAROSE GELS WITH DEAE PAPER

  ISOLATION OF DNA FROM AGAROSE GELS WITH DEAE PAPER MATERIALS: The DEAE paper can be used as supplied by Schleicher and Schuell, however the binding capacity can be increased by washing the paper in 10mM EDTA, pH 7.5 for 10 minutes

DEAE membrane吸附法分离与纯化DNA片段

利用离子交换的原理，在低盐情况下使DNA 吸附在带正电荷的滤膜上，再利用含有高浓度盐类的缓冲液将DNA 自膜上溶离下来。 仪器用具：Mupid II 迷你电泳槽；UV transilluminator;防护面罩；扁平药匙；干净刀片；65℃水浴或恒温槽 药品试剂： 经BamHI 及HindIII 作用的pBlueGus 及pQE31 含EtBr 的1.0 % 12-well agarose gel 1×NET （20 mM Tris-HCl; 150 mM NaCl; 0.1 mM