luteolin-6-C-glucoside-luteoli

ICAM-1纯化 Purification of ICAM-1

Buffer (Tris-saline pH 7.5 @RT {pH 8.0 @ 4o C}) Tris-Base 0.76g NaCl 4.4g dH2 O 450ml Adjust pH to 7.5 with HCl. Add dH2 O to 500ml final volume. Add 0.03% NaN3. Wash Buffer (Tris-saline pH8.0 / 1% Octyl Glucoside

Purification of ICAM-1

to 500ml final volume. Note: Since octyl Glucoside is very expensive, take 100ml of this buffer and add to it 1g of Octyl Glucoside. This will be more than sufficient for one run Equilibrium Buffer (Tris-saline pH 7.5 @RT {pH 8.0 @ 4o C

Glycolipid Binding Assay

MnCl2 ,3 mM PMSF,and 0.1 M Octyl glucoside) 3.Maintain constant agitation for 20 minutes at 4 ℃ . 4.Scrape the cells from the dish and centrifuge(16,000xg,4 ℃ )for 15 minutes,the supernatant is the "total cell lysate". B : Immunoprecipitation 1.Add