Phalloidin-Fluorescein Conjuga

Phalloidin Staining worms

Phalloidin Staining worms by Michael Koelle modified from Beth Bucher and Andrew Chisholm 4/6/94   Phalloidin binds to filamentous actin. This stain allows visualization of mainly of muscles, but some other actin structures

Fluorescein labeling of proteins

This method uses carboxyfluorescein succinimidyl ester (CFSE)rather than fluorescein isothiocyanate,resulting in more reliable labeling.Succinimidyl esters are excellent reagents for amine modification since the amide products formed are very stable

Fluorescein labeling of proteins

on temperature, length of incubation, concentration of protein, concentration of CFSE, and nature of protein. For critical applications, conjugate and test a small amount first to verify the conditions. Separate labeled protein from free Fluorescein