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- APE-C3920-1
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
储存于-20 ° C
- 保质期：
1年
- 英文名：
Palmitoyl Sphingomyelin
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
无
- 规格：
1mg
Palmitoyl Sphingomyelin，Palmitoyl Sphingomyelin，Palmitoyl Sphingomyelin,Palmitoyl Sphingomyelin
Palmitoyl Sphingomyelin-Palmitoyl Sphingomyelin
产品货号：APE-C3920-1
产品规格：1mg
该Palmitoyl Sphingomyelin产品的背景资料具体查看产品。
该Palmitoyl Sphingomyelin产品的产品描述具体查看产品。
Palmitoyl Sphingomyelin-Palmitoyl Sphingomyelin储存于-20 ° C
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文献和实验Purification of Demethylated Sphingomyelin
________________________________________________________________________ 1) Run all three of the above samples on a TLC with the solvent system: chloroform-methanol-ammonium hydroxide (60:35:8). Run sphingomyelin and demethylated sphingomyelin standards as well. 2) Stain with iodine and permanganate.
Labeled Sphingomyelin Synthesis
Synthesis reaction: 1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol. 2) Allow reaction to proceed at room temperature for atleast 18 hours in the dark. 3) Check completion
Sphingomyelin Mass Measurement
Protocol: Bligh & Dyer extraction 1) Pellet approximately 1 X 107 cells. 2) Resuspend pellet in 3 ml CHCl3: CH3OH (1:2) and vortex hard. 3) Add 0.8 ml H2O, vortex hard and allow solution to sit at room temperature for atleast 30
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