Lysine-specific Demethylase In

Extracting DNA Demethylase Activity from Mammalian Cells

Purification of an enzymatic activity requires a simple and relatively expeditious assay of its activity. However, the nature of the demethylase reaction has been elusive for decades. Although a large body of evidence supported the hypothesis

Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells

The mitotic checkpoint prevents cells with unaligned chromosomes from prematurely exiting mitosis by inhibiting the anaphase-promoting complex/cyclosome (APC/C) from targeting key proteins for ubiquitin-mediated proteolysis. Great efforts

A Conformationally Restricted -Strand HIV Protease Inhibitor

cyclopropanes as novel [-NH-Cα-] or [-CO-Cα-] bond replacements in pseudopeptides to restrict both side-chain orientation and enforce backbone secondary structures. To test these assumptions, the cyclopropane containing analog 1 (Fig. 1 ) was modeled