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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
该BL21 Star(DE3) Competent Cell产品的保存方式具体查看产品。
- 保质期:
1年
- 英文名:
BL21 Star(DE3) Competent Cell
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
10X100ul
BL21 Star(DE3) Competent Cell,BL21 Star(DE3) Competent Cell,BL21 Star(DE3) Competent Cell,BL21 Star(DE3) Competent Cell
BL21 Star(DE3) Competent Cell-BL21 Star(DE3) Competent Cell
产品货号:APE-BC2006-1
产品规格:10X100ul
一种高效表达外源非毒性蛋白的感受态,广泛用于T7启动子驱动的重组蛋白表达体系

Cell Biology|Competent Cells

BL21 Star(DE3) Competent Cell-BL21 Star(DE3) Competent Cell该BL21 Star(DE3) Competent Cell产品的保存方式具体查看产品。
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文献和实验CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
(see table). Expression vector appropriate host strain pBAD vectors Top10, LMG194 pET vectors BL21 (DE3) pGEX vectors BL21 pMal vectors BL21, TB1 pProEx vectors BL21, DH10B pQE vectors M15 or M15 [pREP4] pRSET vectors BL21 (DE3) pLysS
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