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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
该BL21(DE3) Competent Cell产品的保存方式具体查看产品。
- 保质期:
1年
- 英文名:
BL21(DE3) Competent Cell
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
10X100ul
BL21(DE3) Competent Cell,BL21(DE3) Competent Cell,BL21(DE3) Competent Cell,BL21(DE3) Competent Cell
BL21(DE3) Competent Cell-BL21(DE3) Competent Cell
产品货号:APE-BC2005-1
产品规格:10X100ul
一种最常用的非毒性蛋白表达感受态,广泛用于T7启动子驱动的重组蛋白表达体系

Cell Biology|Competent Cells

BL21(DE3) Competent Cell-BL21(DE3) Competent Cell该BL21(DE3) Competent Cell产品的保存方式具体查看产品。
点击:BL21(DE3) Competent Cell-BL21(DE3) Competent Cell查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多APExBIO公司特色小分子抑制剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
CCMB buffer to make chemical competent cell
, but you need a big clone or several clones. :D LB media is OK, OD less than 0.8 is OK This protocol has been tested on TOP10, MachI and BL21(DE3) , DH5α cells. 1 Preparing glassware and media 2 Preparing seed stocks 3 Preparing competent cells 4 CCMB80
TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
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