2'-Amino-dCTP-2'-Amino-dCTP

基于PCR技术的染色质沉淀分析

of H2O:54 g of Tris base27.5 g of boric acid20 ml of 0.5 M EDTA (pH 8.0)The 0.5x working solution is 45 mM Tris-borate/1 mM EDTA.TBE is usually made and stored as a 5x or 10x stock solution. The pH of the concentrated stock buffer should be approx

Methods for the Detection of D-Amino-Acid Oxidase

phosphate, and 1 mM EDTA, pH 7.7), 5 x Denhardt's solution ( 8 ), and 0.5% SDS] containing 20 μg/ml denatured, fragmented salmon sperm DNA. The D-amino-acid oxidase cDNA fragment amplified above by PCR was labeled with [-32P]dCTP (~110 TBq/mmol, Amersham

Amino-Allyl cDNA Labeling

RT Master Mix (per sample):             2   µL Superscript II             8   µL 5x 1st strand buffer             0.4 µL 1 M DTT             0.8 µL 50x dNTP mix             4  µL 10x  AA-dUTP  (optional: spike with 1 µL 32 P-dCTP) 5.  Add 15 µL