(-)-Bicuculline methochloride-

[3H]-GABA Binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes

of antagonism by bicuculline and insensitivity to the GABA-analog baclofen, whereas GABAB receptors are stimulated by baclofen and are insensitive to bicuculline; GABAC receptors are insensitive to both (1 ).

Characterization of GABA Receptors

) (–)‐Bicuculline methiodide (Sigma‐Aldrich, or ICN Biomedicals) Muscimol (unlabeled) γ‐Amino‐n ‐butyric

Saturation Assays of Radioligand Binding to Receptors and Their Allosteric Modulatory Sites

. The binding of ligands (e.g., the agonist muscimol and antagonist bicuculline) to the GABA receptor requires both α and β subunits. Similarly, both α and γ subunits are required for optimal binding of the agonist diazepam and the antagonist flumazenil