Sulfo-NHS-Biotin-Sulfo-NHS-Bio

Detection of Cell Surface Dopamine Receptors

and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surface. In the biotinylation method, cell surface receptors are labeled with Sulfo-NHS-biotin. The charge on the sulfonyl

Detection of Tight Junction Barrier Function In Vivo by Biotin

molecules with different sizes have been widely used to determine the TJ barrier function. In the in vivo system, the tracer molecule Sulfo-NHS-Biotin was initially used in Xenopus embryo system and subsequently was successfully applied to a number of animal

生物素标记蛋白操作方法

下面的操作方法可以使约3~5分子的生物素与1分子的蛋白结合，对某些蛋白来说，生物素与蛋白的分子比可根据不同的生物素化要求进行调整。 1. 溶解2~10 mg 蛋白于1 ml 的BuphTm磷酸盐缓冲液中，并计算溶解的毫摩尔数。如果蛋白含有不恰当的缓冲液（如Tris或者甘氨酸），可以通过在PBS中透析或浓缩的方法除去。计算：蛋白质量（mg）/蛋白分子量（MW）＝蛋白质的毫摩尔数。 2. 在打开之前，平衡生物素至室温。加2 mg Sulfo-NHS-Biotin于100 ul