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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 2-8°C
- 保质期:
1年
- 英文名:
Aprotinin
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
10mg
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文献和实验【求助】求助!!关于EMSA核蛋白提取过程中用到的100x Protease Inhibitor
月下独见 我最近打算提核蛋白做EMSA,我是自己配制的buffer,其中要加入100x Protease Inhibitor使buffer里含有1x Protease Inhibitor。这个100x Protease Inhibitor有没有买的?还是要自己配制。 我查了一下资料Protease Inhibitor里含有Aprotinin Leupeptin Pepstatin PMSF,如果自己配Protease Inhibitor的话以上的试剂
TGEK Base 50 mM Tris 10% vol Glycerol 1 mM EDTA 100 mM KCl (add for std lysis buffer): PMSF Benzamidine Leupeptin Aprotinin 0.5% NP40 (add for solubilization buffer) 1% NP40 0.1% Triton X100 0.1% SDS 0.5% NaDeoxycholate Buffer
some of these production systems with respect to their performance: protein yield and quality. Using a specific tester protein (aprotinin), it was shown that functional aprotinin can be manufactured in plants in substantial amounts, as illustrated in this chapter.
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