Large T antigen - rhesus polyo

Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques

Ex vivo enumeration of the absolute frequencies of antigen-specific T cells is key for evaluating the immunogenicity of T cell-based vaccines. Currently there are three methods that are widely used to quantify cellular immune responses

Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques

that can accurately detect even small levels of antigen-specific T-cell responses that may otherwise be missed easily. With this in mind, we developed the dendritic cell enzyme-linked immunospot (DC-ELISPOT) protocol by incorporating antigen-pulsed DCs to stimulate

Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses

Tetrameric major histocompatibility complex (MHC) class I-peptide complexes (tetramers) are a powerful tool in the study of antigen-specific CD8+ T cell responses (1 ,2 ). Although the interaction of a monomeric MHC-peptide complex