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- 南京万木春生物
- 进口/国产
- WM-24JY550
- 2025年12月13日
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- 英文名：
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- 库存：
现货库存
- 供应商：
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- 肿瘤类型：
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- 细胞类型：
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- 品系：
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- 组织来源：
ATCC/DSMZ/ECACC
- 相关疾病：
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- 物种来源：
人或动物
- 免疫类型：
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- 细胞形态：
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- 是否是肿瘤细胞：
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- 器官来源：
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- 运输方式：
常温或干冰
- 年限：
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- 生长状态：
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- 规格：
T25
| 种属 | 小鼠 |
| 组织来源 | 正常肺动脉组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ；生长添加剂5ml ；胎牛血清10ml ；双抗5ml |
| 简介 | 肺动脉是由内膜、中层弹力层和外膜构成 ，三层紧密贴合在一起。其中 ，外膜是专门的支持组织 ，外膜成纤维是外膜 的主要成分 ，在血管炎症反应、血管重塑等方面发挥重要作用。 |
Immunotherapies provide a potential treatment option for currently incurable bone metastases. Bone marrow is an important secondary lymphoid organ with a unique immune contexture. Even at non-disease state immune cells and bone cells interact with each other, bone cells supporting tumors. The FK-PBA targeting overexpressed sialic acid on tumor cells can enable on-demand gelation in residue tumor areas and maintain continuous vaccination in surgical bed. Unlike neoantigen-based vaccine or adoptive cell therapy that takes several months to prepare,
Questions/purposes: Our objectives were (I) characterize the epidemiology of de novo bone metastasis with respect to patient demographics, (II) characterize the incidence by primary site, age, and sex (2010-2015), and (III) compare survival of de novo metastatic cancer patients with and without bone metastasis.
Methods: This is a retrospective, population-based study using nationally representative data from the Surveillance, Epidemiology, and End Results program, 2010-2015. Incidence rates by year of diagnosis, annual percentage changes, Kaplan-Meier, univariate and multiple Cox regression models are included in the analysis.
Results: Of patients with cancer in the SEER database, 5.1% were diagnosed with metastasis to bone, equaling ~18.8 per 100,000 bone metastasis diagnoses in the US per year (2010-2015). For adults >25, lung cancer is the most common primary site (2015 rate: 8.7 per 100,000) with de novo bone metastases, then prostate and breast primaries (2015 rates: 3.19 and 2.38 per 100,000, respectively). For patients <20 years old, endocrine cancers and soft tissue sarcomas are the most common primaries. Incidence is increasing for prostate (Annual Percentage Change (APC) = 4.6%, P < 0.001) and stomach (APC = 5.0%, P = 0.001) cancers. The presence of de novo bone metastasis was associated with a limited reduction in overall survival (HR = 1.02, 95%, CI = [1.01-1.03], p < 0.001) when compared to patients with other non-bone metastases.
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文献和实验/the development of immune cells and immune cells regulating bone turnover. In cancer, tumor cells interfere with this homeostatic process starting from formation of pre-metastatic niche and later supporting growth of bone metastases. In this review, we introduce a novel concept
于 4% 多聚甲醛内4度后固定 4-6 个钟头。 四、难点与关键点 将灌注针插入主动脉内是灌注固定的关键，也是难点。首先准确找到主动脉，这是此步骤的要点。可用温生理盐水将胸腔内的血液冲洗干净，用眼科镊子轻轻夹住心 外膜（夹的越少越好，以免影响取材）将心脏向左上方提起，即可看清主动脉，又可使灌注针很容易地插入主动脉内。 插入时动作要慢，针尖方向不要偏向右侧，以免刺入右心房，如果感到有阻力，则将针退后、调整方向重新进针，直到进入主动脉，灌注针进入主动脉后可在心脏的上方看到其位置，灌注针进入主动脉的长度
liupeizc 请问哪位高手知道成纤维细胞的生长周期啊，更确切的是血管外膜成纤维细胞生长周期，谢谢！ zhujoker 估计都没人做过，你如果需要观察其生物学功能，就自己做一次，也算原创了啊！ freecell 这里有： http://www.currentprotocols.com/protocol/cb0201 本文由丁香园论坛提供，想了解更多有用的、有意
培养基。放入37℃,5%ＣＯ2培养箱中培养。72ｈ后细胞从环内外生长迁出,环内为内皮细胞,环外为成纤维细胞。将动脉环除去后,可看到内膜面细胞集落与外膜面之间有明显的无细胞区界限，用玻璃针剔除成纤维细胞,得到纯的内皮细胞生长克隆。继续培养10～15ｄ，此时FBS浓度为２０％,形成细胞单层。 4、置于5%CO2孵箱中培养，每隔3天换液，培养时添加15% FBS，5～10μg/ml的内皮细胞生长因子（ECGF）以及100μg/ml的肝素。待细胞90%~95%融合时，0.25胰蛋白
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