小鼠胚胎干细胞。该细胞缺乏HGPRT(HPRT),并且对 0.06mM 的 6-硫鸟嘌呤有抗性。当在饲养层(胚胎成纤维细胞或 STO 细胞)上培养时,细胞保持未分化状态。在没有饲养层的情况下,细胞自发分化并形成胚胎结构。当 注入胚泡中时,细胞可以进入生殖系。在常规的分子基因修饰技术之后,它们可用于重构小鼠胚胎。培养时需使用昆明白MEF作为饲养层细胞。
形态
球形克隆
生长特征
贴壁生长
倍增时间
每周2 至3 次
培养条件
气相:空气,95%;二氧化碳,5%。 温度:37摄氏度,培养箱湿度为70%-80%。
冻存条件
冻存液:90%FBS,DMSO10%, 或使用非程序冻存液:官网货号JY-H040
产品使用
仅限于科学研究,不可作为动物或人类疾病的治疗产品使用。
19156044901 (同微信)
landscape of checkpoint blockade but also the way in which immunotherapy trial outcolks are determined. Antibodies targeting PD-1 and its ligand, PD-L1, soon followed and use of these checkpoint inhibitors (ICIs) have expanded exponentially. ICI treatlknt has shown long-lasting clinical benefit in several tumor types and patients
landscape of checkpoint blockade but also the way in which immunotherapy trial outcolks are determined. Antibodies targeting PD-1 and its ligand, PD-L1, soon followed and use of these checkpoint inhibitors (ICIs) have expanded exponentially. ICI treatlknt has shown long-lasting clinical benefit in several tumor types and patients