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文献和实验here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1?1.20.18. © 2012 by John Wiley & Sons
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
precipitate which develops at intermediate rates. The color can be distinguished from the purple color derived from X-Phos + NBT. Adding tetrazolium red or blue makes the product look more purple (less pink). Salmon-phos (Biosynth International) results
How to perform automated counts of fluorescently stained cells.
. In Photoshop, use the marquee tool measure the size of large square of the brightfield hemocytometer image. (A large square on the hemocytometer is 1mm x 1mm x 0.1mm = 0.1 µL) 11. Crop the u.v. fluorescent image of cells to same size as a large square
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