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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
Amersham #55022 Microcon-30 filter 3 Amicon #42410 TE (pH 7.4) ~5 mL polyA DNA or RNA (10 mg/mL in TE) 1.0 uL 20X SSC 3.0 uL
Preparation of Fluorescent DNA Probe from mRNA: Yeast
Preparation of Fluorescent DNA Probe from mRNA: Yeast Last updated: 3/29/99 Materials for 2 reactions (Cy3 & Cy5) Qty Order info Heat blocks, 42C & 70C
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration
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