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文献和实验?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder
Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gone
The green fluorescent protein (GFP; 1 –5 ) from the jellyfish Aequorea Victoria has emerged as an important reporter for monitoring gene expresston, protein localization, cell transformation, and cell lineage in VIVO and in real time
antifade can be stored for up to 3 weeks at 4°C. See note 6 See comment 5 and comment 6. Acquisition Analyze the slides under a fluorescent microscope equipped with a CCD camera, a 63X or a 100X oil objective and appropriate filters
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