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文献和实验antifade can be stored for up to 3 weeks at 4°C. See note 6 See comment 5 and comment 6. Acquisition Analyze the slides under a fluorescent microscope equipped with a CCD camera, a 63X or a 100X oil objective and appropriate filters
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-treatment conditions.Histochem . J. 18 :271. Rodriguez, J., and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12 :316. Rosenberg, W.S., X.O. Breakefield, C. DeAntonio, and O
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
with a suitable fluorescent marker in the target neurons. Image axons at a similar magnification (0.2 um/pixel or higher magnification) and position under the crosshairs (fig.7). 7) Open the laser safety shutter and trigger the ablation laser
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