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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
DTT (0.1 M) 3.0 7.5 10 mM 50X dNTPs 0.6 1.25 500 uM; 200 uM dTTP Cy3- or Cy5 dUTP 3.0
Preparation of Fluorescent DNA Probe from mRNA: Yeast
DNA or RNA (10 mg/mL in TE) 1.0 uL 20X SSC 3.0 uL Millipore filter, 0.45 um 1 Millipore #UFC3OHVNB . *50X dNTPs stock
alone for 1 hour at RT, then add the anti-Biotin and incubate an additional hour at RT with both antibodies; Rinse tissues in PBS-Tr (3x 5-10 minutes each wash); Wash three more times in PBS-Tr, 20 minutes each wash; Counterstain DNA with DAPI
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