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文献和实验Construction and Characterization of Adenovirus Vectors
an extinction coefficient of 1.1 x 1012 for wild-type Ad (Maizel et al. 1968 ), calculate the number of particles/milliliter: (OD260 )(dilution factor)(1.1 x 1012 ). A typical Ad vector preparation should have a ratio of particle to pfu of ~10
Creation and Use of Infectious Virus Vector
for this; polystyrene tubes DO NOT work!). 1 ug retroviral DNA encoding gene X 1 ug packaging plasmid (such as pCLEco, pCLampho, pUMVC, pHR’CVM8.2 deltaR etc.). If you are using a 3 plasmid system then:
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
for the quantitative analysis of protein?protein interactions using the flow cytometry protein interaction assay (FCPIA). This method is based upon immobilizing protein on a polystyrene bead, incubating these beads with a fluorescently labeled binding partner
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