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文献和实验by reaction with alkaline phosphatase-conjugated goat anti-mouse secondary antibody. Choose the actin pool broadly, however, consider also checking the column fractions by dot blot with mAB 3F2 to identify capping protein. Pool the actin fractions
Protein purification: skeletal muscle myosin
consists of an alpha-helical coiled-coil rod of 150 nm in length. The most important mechano-enzyme for muscle contraction. Material Source: rabbit skeletal muscle
Material 1. Source: rabbit skeletal muscle 2. Equipment: • mincer • glass-Teflon-homogenizer with pestle • sephacryl S-300 column (5 x 100 cm) • electrophoretic gels • centrifuge • sterile cheesecloth
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