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文献和实验Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
in a polystyrene cuvette box, cover with a polystyrene lid that fits, secure with tape, and freeze @ -70o C (the temperature optimally should drop approximately 1o C/minute). 10. For samples being screened by PCR, take the plate
-078/Lot #1060198)Fetal Bovine Serum (GibcoBRL-Cat# 10437-028/Lot # 1026566)-filter sterilize (0.22um filter) before mixed into DMEMIsobutylmethylxanthine (IBMX; Sigma I-7018)Dexamethasone (Sigma D-4902)Insulin (Bovine; Sigma I-5500)MEM Sodium Pyruvate
Nick Translation of DNA for CGH
Biotin-dUTP Digox-dUTP FITC-dUTP Texas Red dUTP 10X Biotin dNTP 5 ul 0 ul 0 ul
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