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文献和实验Construction and Characterization of Adenovirus Vectors
. First-generation Ads have a large cloning capacity (5-14 kbp) and efficiently transduce a wide range of both quiescent and proliferating cell types. They are readily propagated to produce high-titer stocks (1011 -1013 vector particles from a 3-L culture
- make a 5 uM stock solution of adaptors - add 5 ul of the top strand adaptor (100 uM) and 5 ul of the bottom strand adaptor (100 uM) to 80 ul H2O + 10 ul 10X BRL buffer 3. c) heat both to boiling, and let cool to RT. Ligation of Adaptors
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
that are subsequently ligated toSplinkBlunt. Those biotin-bearing DNA fragments are then purified with streptavidin magnetic particles prior to tworounds of PCR and products cloning as performed in (a) (Taken from Yin, B. et al., BioTechniques 2007. . 2008BioTechniques
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