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文献和实验Mouse B cell isolation with magnetic beads
separation Positive :3 ml MACS for equilibrium, then cell solution, then wash 3x3ml followed by 2x4ml . Put the column on a 15 ml tube and flush the column with 5ml; Negative : 3 ml MACS for equilibrium, then collect the elution
Magnetic Fluids Their Applications to Medicine
of University College London. Ferro-fluids are stable colloidal suspensions of single domain particles, typically 10nm in diameter, of ferromagnetic or ferrimagnetic materials. The particles are typically Fe3O4 or Fe2O3 produced by co-precipitation:
Methods for the Detection of D-Amino-Acid Oxidase
were homogenized in 7 mM pyrophosphate buffer (pH 8.3). The homogenates were centrifuged at 550 x g for 5 minutes. The supernatant solutions were used for the assay. The reaction mixture consisted of 0.3 ml of 0.133 M pyrophosphate buffer (pH 8.3) containing
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