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文献和实验Construction and Characterization of Adenovirus Vectors
), 1.25 g/mL and 1.35 g/mL, in dialysis buffer Prepare each gradient solution separately. Sterilize by filtration through a 0.2-µm filter. Chloroform:isoamyl alcohol (24:1) Citric saline (2X) For 1X citric saline, dilute the 2X
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
to the digests to drive the primer extension reaction. Pfu DNA polymerase is used to produce blunt-end doublestrandedDNA fragments. The extension products are subsequentlypurified with streptavidin magnetic particles, prior to ligation toSplinkBlunt
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
in preparation). This explains why the GBP does not recognise unfolded or denatured GFP (e.g. on immunoblots). For immunoprecipitations of GFP fusion proteins we coupled the GBP covalently to monovalent matrices (e.g. agarose beads or magnetic particles
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