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文献和实验Functionalized Magnetic Nanoparticles as an In Vivo Delivery System
We developed extremely small functionalized magnetic nanoparticles (MNPs) for use as an in vivo delivery system for pharmaceuticals and biomolecules. We functionalized the MNPs (d = 3 nm) by silanization of amino groups on the particles
Preparation of Para-magnetic beads from Promega cat#Z5482: a) suspend magnetic particles in bottle - transfer 200 ul (200 ug) of beads per sample of RNA to be purified to a microfuge tube or tubes. b) place microfuge tube
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
in preparation). This explains why the GBP does not recognise unfolded or denatured GFP (e.g. on immunoblots). For immunoprecipitations of GFP fusion proteins we coupled the GBP covalently to monovalent matrices (e.g. agarose beads or magnetic particles
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