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- 2026年06月28日
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文献和实验Conjugation of Nanoparticles to Proteins
. First, stable and biocompatible 1.5nm gold nanoparticles are synthesized. Conjugation of the nanoparticle to the protein is then achieved via two different approaches that do not require heavy chemical modifications or cloning: cysteine–gold covalent bonding
Reverse Transfection Using Gold Nanoparticles
, we have found that the conjugation of gold nanoparticles (20 nm in particle size) to the pEGFP-N1/Jet–PEI complex resulted in an increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal
Phycoerythrin conjugation protocol
reagents and incubate at 4 °C overnight on rotary mixer. Add 80 µl N-ethyl maleimide (0.1 mM) to reaction mixture to stop reaction. Agitate and incubate 30 mins at room temperature Concentrate to 0.5 ml or less on Centricon
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