- ￥420 - 2365
- Elabscience
- E-AB-19991
- 湖北武汉
- 2025年07月02日
- IHC;ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
HLA-DO/B Polyclonal Antibody_货号:E-AB-19991_多克隆抗体
- 抗体英文名：
HLA-DO/B Polyclonal Antibody
- 应用范围：
IHC;ELISA
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
12个月
- 目录编号：
E-AB-19991
- 级别：
科研级别
- 库存：
999
- 供应商：
武汉伊莱瑞特生物科技股/份有限公司
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态：
液体
- 亚型：
IgG
- 规格：
200μL/120μL/60μL/20μL
| 规格： | 200μL | 产品价格： | ￥2365.0 |
|---|---|---|---|
| 规格： | 120μL | 产品价格： | ￥1640.0 |
| 规格： | 60μL | 产品价格： | ￥1010.0 |
| 规格： | 20μL | 产品价格： | ￥420.0 |
货号：E-AB-19991
| Verified Samples |
Verified Samples in IHC:Human tonsil
|
| Dilution |
IHC 1:40-1:200, ELISA 1:5000-1:10000
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Synthetic peptide of human HLA-DO/B
|
| Abbre |
HLA-DO/B
|
| Synonyms |
HLA-DO/B;HLA class II histocompatibility antigen;DO beta chain;MHC class II antigen DO/B抗体
|
| Swissprot |
P13765
|
| Cellular Localization |
Endosome membrane,Lysosome,lysosomal membrane,lysosome,Plasma Membrane,MHC class II protein complex,Other locations:integral component of membrane
|
| Concentration |
1.26 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
|
| Purification Method |
Antigen aF/FInity purification
|
| Research Areas |
Immunology
|
| Conjugation |
Unconjugated
|
| Background |
HLA-DO/B belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DO/B), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. HLA-DO/B (Major Histocompatibility Complex, Class II, DO Beta) is a Protein Coding gene. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and CTLA4 Signaling. GO annotations related to this gene include MHC class II protein complex binding and MHC class II receptor activity. An important paralog of this gene is HLA-DRB1.
|
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Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Construction of Polyclonal Antibody Libraries Using Phage Display
Polyclonal antibody libraries (PCALs) are standardized mixtures of antibodies (Abs) specific for an antigen (Ag) or multi-Ag target (a poly-Ag). As the immunoglobulin (Ig) genes are cloned, the mixtures can be perpetuated, amplified
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