SMAD2 Polyclonal Antibody 货号：E

Verified Samples in WB: HT29, NIH/3T3, Mouse heart, Mouse liver, Mouse brain, Rat heart, Rat liver, Rat brain
Verified Samples in IHC: Mouse heart, Rat skeletal muscle

WB 1:500-1:2000, IHC 1:300-1:800

Polyclonal

IgG

Recombinant protein corresponding to MouseSmad2

SMAD2

Drosophila, HsMAD2, JV18, JV18-1, JV181, MAD, MAD Related Protein 2, MAD homolog 2, MADH2, MADR2, MGC22139, MGC34440, Mad-related protein 2, Mother against DPP homolog 2, Mothers against decapentaplegic, Mothers against decapentaplegic homolog 2, hMAD-2, homolog of

Q15796, Q62432, O70436

60 kDa

60 kDa

Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.

500 μg/mL

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH 7.4.

Affinity purification

Cancer, Epigenetics and Nuclear Signaling, Signal Transduction, Stem Cells

Unconjugated

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.