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- 文献和实验
- 技术资料
- 抗体名:
Histone H3 Polyclonal Antibody_货号:E-AB-70330_多克隆抗体
- 抗体英文名:
Histone H3 Polyclonal Antibody
- 靶点:
Histone H3
- 浓度:
5 μL/Test
- 应用范围:
WB
- 宿主:
Rabbit
- 适应物种:
Human;Mouse;Rat
- 保质期:
12个月
- 级别:
科研
- 库存:
99
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 保存条件:
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
- 形态:
液体
- 亚型:
IgG
- 规格:
200μL/120μL/60μL
| 规格: | 200μL | 产品价格: | ¥3300.0 |
|---|---|---|---|
| 规格: | 120μL | 产品价格: | ¥2220.0 |
| 规格: | 60μL | 产品价格: | ¥1380.0 |
货号:E-AB-70330
| Verified Samples |
Verified Samples in WB:Hela,Jurkat,HepG2,Tumor of Hela,Mouse spleen,Mouse lung,Mouse kidney,Rat spleen,Rat lung
|
| Dilution |
WB 1:300-1:1000
|
| Clonality |
Polyclonal
|
| Isotype |
IgG
|
| Immunogen |
Recombinant protein corresponding to Mouse Histone H3
|
| Abbre |
Histone H3
|
| Synonyms |
H3 histone family;member A;H3/A;H31;H3FA;Hist1h3a;HIST1H3B;HIST1H3C;HIST1H3D;HIST1H3E;HIST1H3F;HIST1H3G;HIST1H3H;HIST1H3I;HIST1H3J;histone 1;H3a;Histone cluster 1;H3a;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l抗体
|
| Swissprot |
P68431,P68433,Q6LED0
|
| Calculated MW |
15kDa
|
| Observed MW |
15-17kDa
|
| Cellular Localization |
Chromosome, Nucleosome core, Nucleus
|
| Concentration |
1.1 mg/mL
|
| Storage |
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
|
| Buffer |
PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
|
| Purification Method |
Affinity purification
|
| Research Areas |
Cancer; Epigenetics and Nuclear Signaling
|
| Conjugation |
Unconjugated
|
| Background |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
|
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文献和实验Near infrared-responsive quinacrine–gold hybrid nanoparticles deregulate HSP-70/P300-mediated H3K14 acetylation in ER/PR+ breast cancer stem cells
IF:4.7
Journal:Nanomedicine
DOI:10.2217/nnm-2023-0269
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
Genome-Wide Measurement of Histone H3 Replacement Dynamics in Yeast
chromatin. Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis. Here, we describe a protocol for measuring histone replacement rates genome wide
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
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