AE93311Hu 人八聚体转录因子(OTF2A)ELISA

人工转录因子的部件――人类锌指结构

of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP

利用人工组合转录因子对人类基因组扫描

specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority

HDAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方案

DAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方组蛋白去乙酰化酶 （histone deacetylase,HDAC ）是一类蛋白酶,对染色体的结构修饰和基因表达调控发挥着重要的作用。一般情况下,组蛋白的乙酰化有利于DNA与组蛋白八聚体的解离，核小体结构松弛，从而使各种转录因子和协同转录因子能与DNA结合位点特异性结合，激活基因的转录。在细胞核内，组蛋白乙酰化与组蛋白去乙酰化过程处于动态平衡，并由组蛋白乙酰化转移 酶（histone