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文献和实验, estimated price $600-$900)。 ELISA-BASED ASSAYS(ELISA分析) What they are:基于酶联免疫反应的转录因子结合分析。 What to use them for:定量转录因子的浓度。 Pros(优点):具有快速,定量和高通量的特点。 Cons(缺点):需要目的转录因子的一种抗体才能进行试验。 Things to keep in mind:ELISA-based assays可以比传统的凝胶迁移分析
of VEGF protein secreted from HEK 293 cells that had been transiently transfected with the corresponding ZFPs; measured with an ELISA assay kit (Chemicon, Temecula, CA). 'Vector' indicates a control vector that encode no ZFPs. An 'irrelevant ZFP
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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