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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
Rat IgG2b, κ
- 形态:
液体
- 保存条件:
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
- 克隆性:
Monoclonal
- 标记物:
PE/Cyanine5.5
- 适应物种:
Mouse
- 保质期:
12个月
- 目录编号:
E-AB-F0997UI
- 级别:
科研
- 库存:
999
- 供应商:
武汉伊莱瑞特生物科技股份有限公司
- 宿主:
Rat
- 应用范围:
FCM
- 抗体英文名:
PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody[2.4G2]_货号:E-AB-F0997UI
- 抗体名:
PE/Cyanine5.5 Anti-Mouse CD16/32 Antibody流式抗体[2.4G2]_货号:E-AB-F0997UI
- 规格:
100μg/25μg
| 规格: | 100μg | 产品价格: | ¥2280.0 |
|---|---|---|---|
| 规格: | 25μg | 产品价格: | ¥720.0 |
货号:E-AB-F0997UI
| Background |
CD16 is low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses.
|
| Alternate Names |
CD16a/b;CD32;CD32A/B;Fc fragment of IgG low affinity IIa/b receptor;Fc fragment of IgG low affinity IIIa/b receptor;Fc fragment of IgG low affinity IIIb receptor;Fc gamma receptor III A/B;FCG2A;FcGR;FCGR2A/BFCGR3;FCGR3A/B;Fc gamma RIIa/b ;
|
| Swissprot |
P08508;P08101;
|
| Gene ID |
14130,14131
|
| Clone No |
2.4G2
|
| Host |
Rat
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Rat IgG2b, κ
|
| Isotype Control |
PE/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2]
|
| Conjugation |
PE/Cyanine5.5
|
| Conjugation Information |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验自2012年第一篇引用文献问世,至2023年8月,Elabscience已协助各地科研工作者发表超13,000篇SCI文献,包括行业顶/级期刊Nature Medicine, Nature, Cell, Signal Transduction and Targeted Therapy, Circulation, Molecular Cancer, Immunity, Advanced Materials等,总影响因子(IF)59472.59,其中IF≥30的文献超30篇,最高影响因子为66.849,发文单位包含北京大学、中国科学技术大学、浙江大学、斯坦福大学、耶鲁大学、柏林夏里特大学等国内外各大高校。
每年引用文献数量持续增长,保持逐年30%+高增长率,可以预见,Elabscience产品将帮助更多科研工作者发表高质量SCI论文。
文献详情查看>>>>


抗[E-AB-F0997A]进行封闭:往 100 μL(细胞数1×106)细胞悬液中加入 1 μg 纯抗,室温封闭 15 min,直接加入流式抗体进行后续实验。 巨噬细胞检测应尽量避免使用含花青素的流式抗体(如 PE-Cy7),会增加非特异性染色。若为非巨噬细胞检测,使用含花青素的流式抗体(如 PE-Cy7)时也需要封闭。
#01 什么是同型对照? 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照? 流式抗体与靶标的结合,是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞(除T细胞外)表面大多表达 CD16、CD32、CD64 等 Fc 受体,这些受体可与各种抗体的 Fc 端非特异性结合,出现非特异
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
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