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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
40 1 Rail carrier for X26, square 40mm length Newport CN26-40 4
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
for 14 days at 37°C. 11. Between 3 and 7 days and 10 and 14 days incubation, subculture 0.1 ml of test broth ontoan agar plate and incubate plate anaerobically as above. 12. Observe agar plates after 14 days incubation at x300 magnification using
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