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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
Procedure for GenEluteTM Plasmid Miniprep Kit（质粒小量提取）
: When working with bacterial strains containing the wild-type EndA+ gene,such as HB101,JM101,and the NM and PR series,the Optional Wash step is necessary to avoid nuclease contamination of the final plasmid DNA product. 7.Wash column Important Reminder: Verify
Concentration Module 1) To the sample from Step 5, above, add 250 μl Binding Buffer (L3) and 125 μl 96-100% ethanol. Mix well by vortexing. 2) Bind the sample from Step 1 containing Binding Buffer (L3) and ethanol to the spin column
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