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文献和实验AA Metabolite Quantitation of Media Post-AA Labeling
/2,2,4-trimethylpentane/acetic acid/ H2O at a ratio of 90:50:20:100 SYSTEM #2 --> Separates DAG & AA. benzene/diethyl ether/ethanol/NH3 at a ratio of 100:80:4:0.2 B) Following TLC remove plates and allow to evaporate completely. This should take a few hours
AA Metabolite Quantitation of Cell Pellets Post-AA Labeling
. SYSTEM #1 --> SeparatesPA, TXB2, HHT, HETE & AA. ethylacetate/2,2,4-trimethylpentane/acetic acid/ H2O at a ratio of 90:50:20:100 SYSTEM #2 --> Separates DAG & AA. benzene/diethyl ether/ethanol/NH3 at a ratio of 100:80:4:0.2 B) Following TLC remove plates
HC Dropout Plate Supplementation Table Amino Acid Stock Amount To Spread (per 25 ml plate) URA 1 mg/ml 875 µl ADE
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