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文献和实验Table F.1. Rotor Characteristics Figure F.1. Cross section of Sorvall SS-34 rotor. Rotors for a centrifuge are either fixed angles , swinging buckets , continuous flow, or zonal, depending upon whether the sample is held at a given angle
Clonality - X Chromosome Inactivation Assay
microdissected cells in 20 µl proteinase K buffer. Incubate overnight at 37°C. B: DNA Purification Lyse the cells overnight at 42°C, in 50 µl phenol:chloroform:isoamyl alcohol. Boil 10 min at 95°C. Vortex 1 min. Centrifuge 20 min at 14,000 rpm at RT
to be able to compare one rotor's design characteristics with those of a differing rotor. A procedure may be given as centrifuging for 20 minutes at 15,000 RPM in a Sorvall SS34 rotor. The problem then becomes one of how do you equate that to a Beckman JA-20
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